GtCCR1_EYFP_pcDNA3.1
(Plasmid
#159120)
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PurposeThis plasmid encodes GtCCR1 rhodopsin domain
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 159120 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3.1(+)
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGtCCR1
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Alt nameKU761994
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SpeciesGuillardia theta
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Insert Size (bp)900
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Tag
/ Fusion Protein
- EYFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GtCCR1_EYFP_pcDNA3.1 was a gift from John Spudich (Addgene plasmid # 159120 ; http://n2t.net/addgene:159120 ; RRID:Addgene_159120) -
For your References section:
Bacteriorhodopsin-like channelrhodopsins: Alternative mechanism for control of cation conductance. Sineshchekov OA, Govorunova EG, Li H, Spudich JL. Proc Natl Acad Sci U S A. 2017 Nov 7;114(45):E9512-E9519. doi: 10.1073/pnas.1710702114. Epub 2017 Oct 25. 10.1073/pnas.1710702114 PubMed 29078348
