pKLV-EF1amCherry-2A-OsTIR1-W
(Plasmid
#159296)
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PurposeLentiviral vector expressing mCherry and OsTIR1 driven by human EF1a promoter for the auxin-inducible degron (AID) system
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 159296 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepKLV-EF1amCherry
- Backbone size w/o insert (bp) 8811
- Total vector size (bp) 10539
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOsTIR
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Insert Size (bp)1728
- Promoter human EF1a promoter
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer EF1a-seqU1 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKLV-EF1amCherry-2A-OsTIR1-W was a gift from Kosuke Yusa (Addgene plasmid # 159296 ; http://n2t.net/addgene:159296 ; RRID:Addgene_159296)