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(Plasmid #159455)


Item Catalog # Description Quantity Price (USD)
Plasmid 159455 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Invitrogen/Thermofisher Scientific
  • Backbone size w/o insert (bp) 5065
  • Total vector size (bp) 7343
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin, Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    Entacmaea quadricolor
  • Insert Size (bp)
  • GenBank ID
  • Promoter CMV
  • Tag / Fusion Protein
    • mTagBFP2 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TAATACGACTCACTATAGG
  • 3′ sequencing primer ATTTAGGTGACACTATAG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FRT LAMP3 mCRISPRed_mTagBFP2 pcDNA5 was a gift from Oliver Griesbeck (Addgene plasmid # 159455 ; ; RRID:Addgene_159455)
  • For your References section:

    Targeted In Situ Protein Diversification and Intra-organelle Validation in Mammalian Cells. Erdogan M, Fabritius A, Basquin J, Griesbeck O. Cell Chem Biol. 2020 May 21;27(5):610-621.e5. doi: 10.1016/j.chembiol.2020.02.004. Epub 2020 Mar 5. 10.1016/j.chembiol.2020.02.004 PubMed 32142629