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Addgene

pZmSWEET13a:ZmSWEET13a:GUS
(Plasmid #159535)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159535 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pGoldenGate-AG
  • Backbone size w/o insert (bp) 8469
  • Total vector size (bp) 19028
  • Modifications to backbone
    none
  • Vector type
    Bacterial Expression, Plant Expression
  • Selectable markers
    Basta

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ZmSWEET13a
  • Alt name
    ZmSWT13a
  • Species
    Z. mays
  • Insert Size (bp)
    10556
  • GenBank ID
    NM_001155615
  • Promoter native SWEET13a promoter
  • Tag / Fusion Protein
    • GUS (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GACGACAGATCGATCAACCCAAT
  • 3′ sequencing primer TCGTACCACTTCTCTTCCAGTC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2020.09.06.284943 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZmSWEET13a:ZmSWEET13a:GUS was a gift from Wolf Frommer (Addgene plasmid # 159535 ; http://n2t.net/addgene:159535 ; RRID:Addgene_159535)

  • For your References section:

    Evidence for phloem loading via the abaxial bundle sheath cells in maize leaves. Bezrutczyk M, Zollner NR, Kruse CPS, Hartwig T, Lautwein T, Kohrer K, Frommer WB, Kim JY. Plant Cell. 2021 May 5;33(3):531-547. doi: 10.1093/plcell/koaa055. 10.1093/plcell/koaa055 PubMed 33955497
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