pPB-CAG-Brainbow3.2-PGK-PuroTK
(Plasmid #159928)

• Purpose: Brainbow transgenesis
• Depositing Lab: Hideyuki Okano
• Depositing Organization: Keio University
  Located in Japan
• Publication: Yoshimatsu et al Stem Cell Res. 2021 Jan 8;51:102164. doi: 10.1016/j.scr.2021.102164.
• Citations: 2 Articles

Backbone

• Vector backbone: pPB-MCSfwd
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: NFPnls
• Species: Synthetic
• Insert Size (bp): 705

Gene/Insert 2

• Gene/Insert name: mOrange2
• Species: Synthetic
• Insert Size (bp): 708

Gene/Insert 3

• Gene/Insert name: EGFP
• Species: Synthetic
• Insert Size (bp): 717

Gene/Insert 4

• Gene/Insert name: mKate2
• Species: Synthetic
• Insert Size (bp): 696

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Joshua Sanes

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Brainbow3.2 cassette was derived from pThy1-Brainbow3.2 (https://www.addgene.org/45179/)

Addgene QC identified several ambiguous bases in the backbone sequence that could not be resolved by standard NGS. These ambiguities are present in the backbone sequence and are not of concern for the function of the construct.

DNA (Catalog # 159928-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 159928-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.