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T7pr-His6-MBP-TEV-dCas9-BirA*
(Plasmid #159994)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 159994 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pML-2CT
  • Backbone manufacturer
    MacroLab, UC Berkeley
  • Backbone size w/o insert (bp) 4766
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9-BirA*
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    6492
  • Mutation
    D10A, H840A
  • Promoter T7
  • Tag / Fusion Protein
    • 6X-His, MBP, 3X-FLAG, NLS (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 Terminal
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    T7pr-His6-MBP-TEV-dCas9-BirA* was a gift from Jacob Corn (Addgene plasmid # 159994 ; http://n2t.net/addgene:159994 ; RRID:Addgene_159994)
  • For your References section:

    The Histone Chaperone FACT Induces Cas9 Multi-turnover Behavior and Modifies Genome Manipulation in Human Cells. Wang AS, Chen LC, Wu RA, Hao Y, McSwiggen DT, Heckert AB, Richardson CD, Gowen BG, Kazane KR, Vu JT, Wyman SK, Shin JJ, Darzacq X, Walter JC, Corn JE. Mol Cell. 2020 Jul 16;79(2):221-233.e5. doi: 10.1016/j.molcel.2020.06.014. Epub 2020 Jun 29. 10.1016/j.molcel.2020.06.014 PubMed 32603710