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pAAV-hsyn-FLPo-T2A-GFP
(Plasmid #161766)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 161766 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV-hSynapsin
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 4525
  • Total vector size (bp) 6591
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    FLPo
  • Species
    FLPo is a mouse codon-optimized FLP
  • Promoter hSynapsin1

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer hSyn1pro-F
  • 3′ sequencing primer WPRE-R
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    T2AEGFP
  • Species
    Aequorea victoria
  • Promoter hSynapsin1

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer hSyn1pro-F
  • 3′ sequencing primer WPRE-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hsyn-FLPo-T2A-GFP was a gift from Baoji Xu (Addgene plasmid # 161766 ; http://n2t.net/addgene:161766 ; RRID:Addgene_161766)
  • For your References section:

    TrkB-expressing paraventricular hypothalamic neurons suppress appetite through multiple neurocircuits. An JJ, Kinney CE, Tan JW, Liao GY, Kremer EJ, Xu B. Nat Commun. 2020 Apr 7;11(1):1729. doi: 10.1038/s41467-020-15537-w. 10.1038/s41467-020-15537-w PubMed 32265438