pAAV-hsyn-FLPo-T2A-GFP
(Plasmid #161766)

• Purpose: express FLPo and GFP under the control of human synapsin promoter
• Depositing Lab: Baoji Xu
• Publication: An et al Nat Commun. 2020 Apr 7;11(1):1729. doi: 10.1038/s41467-020-15537-w.

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Backbone

• Vector backbone: pAAV-hSynapsin
• Backbone manufacturer: Addgene
• Backbone size w/o insert (bp): 4525
• Total vector size (bp): 6591
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: FLPo
• Species: FLPo is a mouse codon-optimized FLP
• Promoter: hSynapsin1

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: hSyn1pro-F
• 3′ sequencing primer: WPRE-R

Gene/Insert 2

• Gene/Insert name: T2AEGFP
• Species: Aequorea victoria
• Promoter: hSynapsin1

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: hSyn1pro-F
• 3′ sequencing primer: WPRE-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pAAV-hSynapsin-Flpo addgene Plasmid #60663

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry