pAAV-Ef1a-fDIO-mCherryP2ACre
(Plasmid #161773)

• Purpose: Flp-Dependent mCherry and Cre expression
• Depositing Lab: Baoji Xu
• Publication: An et al Nat Commun. 2020 Apr 7;11(1):1729. doi: 10.1038/s41467-020-15537-w.

Backbone

• Vector backbone: AAV
• Backbone manufacturer: Addgene
• Backbone size w/o insert (bp): 5600
• Total vector size (bp): 7411
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: mCherry-P2A-Cre
• Species: Aequorea victoria, bacteriophage P1
• Insert Size (bp): 1805
• Promoter: Ef1a

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ASCI (destroyed during cloning)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: EF1a-F
• 3′ sequencing primer: WPRE-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pAAV-Ef1a-fDIO EYFP (Plasmid #55641)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry