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pLenti-gfaABC1D-monSTIM1
(Plasmid #161797)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 161797 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLenti-EF1a-hChR2(H134R)-EYFP-WPRE
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EGFP-CRY2PHR(mon)-STIM1
  • Alt name
    monSTIM1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3702
  • Entrez Gene
    STIM1 (a.k.a. D11S4896E, GOK, IMD10, STRMK, TAM, TAM1)
  • Promoter gfaABC1D
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer acagcagagatccagtttggtt
  • 3′ sequencing primer TTGCTCACCATGGTGGCGGA
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-gfaABC1D-monSTIM1 was a gift from Won Do Heo (Addgene plasmid # 161797 ; http://n2t.net/addgene:161797 ; RRID:Addgene_161797)
  • For your References section:

    Non-invasive optical control of endogenous Ca(2+) channels in awake mice. Kim S, Kyung T, Chung JH, Kim N, Keum S, Lee J, Park H, Kim HM, Lee S, Shin HS, Do Heo W. Nat Commun. 2020 Jan 10;11(1):210. doi: 10.1038/s41467-019-14005-4. 10.1038/s41467-019-14005-4 PubMed 31924789