LR (bact: HyPer)
(Plasmid
#162069)
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PurposeUsed for the diffuse visualization of H2O2 production in cells.
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Depositing Lab
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Depositing OrganizationMemorial Sloan-Kettering Cancer Center
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 162069 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 162069-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 162069-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepDestTol2pA
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 7903
- Total vector size (bp) 13128
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Vector typeGateway Cloning
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namebeta actin 2
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Alt namebactin2
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SpeciesD. rerio (zebrafish)
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GenBank ID57935
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Entrez Geneactb2 (a.k.a. actbb, bact2, bactin2)
- Promoter T3
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Tag
/ Fusion Protein
- HyPer (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer ATTB1
- 3′ sequencing primer ATTB3
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 162069-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 162069-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LR (bact: HyPer) was a gift from Philipp Niethammer (Addgene plasmid # 162069 ; http://n2t.net/addgene:162069 ; RRID:Addgene_162069) -
For your References section:
Tissue damage detection by osmotic surveillance. Enyedi B, Kala S, Nikolich-Zugich T, Niethammer P. Nat Cell Biol. 2013 Sep;15(9):1123-30. doi: 10.1038/ncb2818. Epub 2013 Aug 11. 10.1038/ncb2818 PubMed 23934216
