LR (bact: HyPer)
(Plasmid
#162069)
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PurposeUsed for the diffuse visualization of H2O2 production in cells.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 162069 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDestTol2pA
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 7903
- Total vector size (bp) 13128
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Vector typeGateway Donor Vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namebeta actin 2
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Alt namebactin2
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SpeciesD. rerio (zebrafish)
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GenBank ID57935
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Entrez Geneactb2 (a.k.a. actbb, bact2, bactin2)
- Promoter T3
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Tag
/ Fusion Protein
- HyPer (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer ATTB1
- 3′ sequencing primer ATTB3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LR (bact: HyPer) was a gift from Philipp Niethammer (Addgene plasmid # 162069 ; http://n2t.net/addgene:162069 ; RRID:Addgene_162069) -
For your References section:
Tissue damage detection by osmotic surveillance. Enyedi B, Kala S, Nikolich-Zugich T, Niethammer P. Nat Cell Biol. 2013 Sep;15(9):1123-30. doi: 10.1038/ncb2818. Epub 2013 Aug 11. 10.1038/ncb2818 PubMed 23934216
