pOX + hSlo2.2
(Plasmid #16215)

• Depositing Lab: Larry Salkoff
• Publication: Slo2.2 (unpublished)

Backbone

• Vector backbone: pOX
• Backbone size w/o insert (bp): 3200
• Vector type: Xenopus Oocyte expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: KCNT1 potassium channel, subfamily
• Alt name: hSlo2.2
• Alt name: GI:73611943
• Species: H. sapiens (human)
• Insert Size (bp): 4600
• Mutation: This construct lacks the first 53aa when compared to GenBank reference sequence
• GenBank ID: NM_020822
• Entrez Gene: KCNT1 (a.k.a. DEE14, EIEE14, ENFL5, KCa4.1, SLACK, Slo2.2, bA100C15.2)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nhe1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that this plasmid does not contain the full-length hSlo2.2 (KCNT1) sequence. When compared to GenBank reference sequence NM_020822, the hSlo2.2 insert in this plasmid is missing the first 159 nucleotides of the coding region, which represent the first 53 amino acids of the hSlo2.2 protein sequence.