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pGEX-4T MDM2 WT
(Plasmid #16237)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16237 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX-4T-3
  • Backbone manufacturer
    Pharmacia
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MDM2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • Entrez Gene
    MDM2 (a.k.a. ACTFS, HDMX, LSKB, hdm2)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX5'
  • 3′ sequencing primer pGEX3'
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T MDM2 WT was a gift from Mien-Chie Hung (Addgene plasmid # 16237 ; http://n2t.net/addgene:16237 ; RRID:Addgene_16237)
  • For your References section:

    HER-2/neu induces p53 ubiquitination via Akt-mediated MDM2 phosphorylation. Zhou BP, Liao Y, Xia W, Zou Y, Spohn B, Hung MC. Nat Cell Biol. 2001 Nov . 3(11):973-82. 10.1038/ncb1101-973 PubMed 11715018