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(Plasmid #162602)


Item Catalog # Description Quantity Price (USD)
Plasmid 162602 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Yeast Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Tef1-Cas9 with RPL25 Intron
  • gRNA/shRNA sequence
  • Species

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This vector was designed as part of a CRISPR course. Please see the following link for an annotated sequence map from the depositing laboratory:

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pG1 was a gift from Lars Steinmetz (Addgene plasmid # 162602 ; ; RRID:Addgene_162602)
  • For your References section:

    CRISPR-Cas9 Gene Editing in Yeast: A Molecular Biology and Bioinformatics Laboratory Module for Undergraduate and High School Students. Sankaran SM, Smith JD, Roy KR. J Microbiol Biol Educ. 2021 May 31;22(2):e00106-21. doi: 10.1128/jmbe.00106-21. eCollection 2021 Fall. 10.1128/jmbe.00106-21 PubMed 34594460