PurposepCCM reconstructed after selection for CCMB1 growth on glycerol under ambient air
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||162709||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepFA31 (derived from pZA31 expressing under PLtet0-1 and constitutive tetR)
- Backbone size w/o insert (bp) 2980
- Total vector size (bp) 14585
Modifications to backboneThe p15A origin was replaced with high-copy colE1 origin.
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
Copy numberHigh Copy
Gene/Insert nameSecond CCM operon cloned from H. neapolitanus
Insert Size (bp)11605
Mutationp15A origin replaced with high-copy colE1 origin
- Promoter PLtet0-1 promoter
- Cloning method Gibson Cloning
- 5′ sequencing primer ggaacctcttacgtgccgatc
- 3′ sequencing primer GCGTTCACCGACAAACAACA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
pCCM reconstructed after selection for CCMB1 growth on glycerol under ambient air. Please note that the I145V mutation in TetR does not impact plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCCM’ was a gift from David Savage (Addgene plasmid # 162709 ; http://n2t.net/addgene:162709 ; RRID:Addgene_162709)
For your References section:Functional reconstitution of a bacterial CO2 concentrating mechanism in E. coli. Flamholz AI, Dugan E, Blikstad C, Gleizer S, Ben-Nissan R, Amram S, Antonovsky N, Ravishankar S, Noor E, Bar-Even A, Milo R, Savage D. Elife. 2020 Oct 21;9. pii: 59882. doi: 10.7554/eLife.59882. 10.7554/eLife.59882 PubMed 33084575