PurposeMammalian expression of rat BDNF
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||162718||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBeta Actin-Halo
Vector typeMammalian Expression
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameBrain derived neurotrophic factor
SpeciesR. norvegicus (rat)
- Promoter Chicken Beta actin
/ Fusion Protein
- Halo (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
pBa vector is susceptible to recombination and should not be grown in fast growing bacteria or cloned using recombination.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBa. BDNF-Halo was a gift from Marvin Bentley (Addgene plasmid # 162718 ; http://n2t.net/addgene:162718 ; RRID:Addgene_162718)
For your References section:A Novel Labeling Strategy Reveals That Myosin Va and Myosin Vb Bind the Same Dendritically Polarized Vesicle Population. Frank M, Citarella CG, Quinones GB, Bentley M. Traffic. 2020 Sep 21. doi: 10.1111/tra.12764. 10.1111/tra.12764 PubMed 32959500