Axin GID 5-6/pCS2MT
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16301||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4352
Vector typeVertebrate expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameAxin GID 5-6
SpeciesX. laevis (frog)
Insert Size (bp)78
MutationGSK-3 interaction domain 380-404
Entrez Geneaxin1.L (a.k.a. XELAEV_18045581mg, axin1, fused)
/ Fusion Protein
- 5x myc (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
GID=Axin GSK3 interaction domain. GID5-6 is a smaller version than GID1-2. These constructs bind and inhibit GSK-3 and potently activate Wnt signaling. For sense transcripts, cut with NotI and transcribe with SP6
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Axin GID 5-6/pCS2MT was a gift from Peter Klein (Addgene plasmid # 16301 ; http://n2t.net/addgene:16301 ; RRID:Addgene_16301)
For your References section:Regulation of glycogen synthase kinase 3beta and downstream Wnt signaling by axin. Hedgepeth CM, Deardorff MA, Rankin K, Klein PS. Mol Cell Biol. 1999 Oct . 19(10):7147-57. PubMed 10490650
Map uploaded by the depositor.