PurposeA plasmid with IFP2.0 coding sequence coded into it. Expresses IFP2.0 with N-term His-tag.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||163124||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5441
- Total vector size (bp) 6140
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)966
/ Fusion Protein
- His (N terminal on backbone)
- Cloning method Gibson Cloning
- 5′ sequencing primer ATGCCATAGCATTTTTATCC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBAD_IPF2.0 was a gift from Julie Biteen (Addgene plasmid # 163124 ; http://n2t.net/addgene:163124 ; RRID:Addgene_163124)
For your References section:Imaging living obligate anaerobic bacteria with bilin-binding fluorescent proteins. Chia HE, Zuo T, Koropatkin NM, Marsh ENG, Biteen JS. Curr Res Microb Sci. 2020 Sep;1:1-6. doi: 10.1016/j.crmicr.2020.04.001. Epub 2020 May 11. 10.1016/j.crmicr.2020.04.001 PubMed 33313576