pLV-PGK-dt/CBA-EGFP-NLS
(Plasmid
#163918)
-
PurposeContains bidirectional reporter genes dtomato and nuclear-targeting EGFP.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 163918 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
-
Vector backbonepLenti6/V5-GW/lacZ
-
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namedtomato and EGFP
-
SpeciesSynthetic
-
MutationAdded nuclear localization signal to EGFP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pLV-PGK-dt/CBA-EGFP-NLS was a gift from John Engelhardt (Addgene plasmid # 163918 ; http://n2t.net/addgene:163918 ; RRID:Addgene_163918) -
For your References section:
Detargeting Lentiviral-Mediated CFTR Expression in Airway Basal Cells Using miR-106b. Choi SH, Reeves RE, Romano Ibarra GS, Lynch TJ, Shahin WS, Feng Z, Gasser GN, Winter MC, Evans TIA, Liu X, Luo M, Zhang Y, Stoltz DA, Devor EJ, Yan Z, Engelhardt JF. Genes (Basel). 2020 Oct 6;11(10). pii: genes11101169. doi: 10.3390/genes11101169. 10.3390/genes11101169 PubMed 33036232