|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16424||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript SK-
- Backbone size w/o insert (bp) 3000
Vector typeIn Situ Template
Growth in Bacteria
Copy numberLow Copy
Gene/Insert namePlexin D1
SpeciesM. musculus (mouse)
Insert Size (bp)1200
Entrez GenePlxnd1 (a.k.a. 6230425C21Rik, b2b1863Clo, b2b553Clo)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
Antisense probes are generated by digesting with EcoR1 and transcribing with T7 RNA Polymerase.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBS-mPlexinD1 was a gift from Jonathan Epstein (Addgene plasmid # 16424 ; http://n2t.net/addgene:16424 ; RRID:Addgene_16424)
For your References section:PlexinD1 and semaphorin signaling are required in endothelial cells for cardiovascular development. Gitler AD, Lu MM, Epstein JA. Dev Cell. 2004 Jul . 7(1):107-16. 10.1016/j.devcel.2004.06.002 PubMed 15239958