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Addgene

VN-dCasE-VC-GK
(Plasmid #164277)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164277 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pEGFP-N1 （EGFP gene knockout）
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VN-dCasE-VC
  • Species
    H. sapiens (human); Escherichia coli
  • Mutation
    H20A in EcCasE
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/635912 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VN-dCasE-VC-GK was a gift from Chunyu Han (Addgene plasmid # 164277 ; http://n2t.net/addgene:164277 ; RRID:Addgene_164277)

  • For your References section:

    A Cas6-based RNA tracking platform functioning in a fluorescence-activation mode. Gao F, Zheng K, Li YB, Jiang F, Han CY. Nucleic Acids Res. 2022 May 6;50(8):e46. doi: 10.1093/nar/gkac014. 10.1093/nar/gkac014 PubMed 35061906
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