pCS2z-etsrp
(Plasmid #164655)

• Purpose: for the synthesis of zebrafish etsrp mRNA
• Depositing Lab: Didier Stainier
• Publication: Mattonet et al Sci Adv. 2022 Sep 2;8(35):eabn2082. doi: 10.1126/sciadv.abn2082. Epub 2022 Aug 31.

Backbone

• Vector backbone: pCS2z
• Backbone manufacturer: addgene #62214
• Backbone size w/o insert (bp): 4292
• Total vector size (bp): 5393
• Vector type: mRNA synthesis

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: etsrp
• Alt name: etv2
• Species: D. rerio (zebrafish)
• Insert Size (bp): 1101
• Entrez Gene: etsrp (a.k.a. ets1b, etv2, grc, wu:fc49b07, zgc:153819)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T3

Resource Information

• Supplemental Documents:
  • pCS2z-etsrp.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry