pCB301TOR-GFP
(Plasmid
#164799)
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PurposeExpression of GFP in oomycetes using Agrobacterium-mediated transformation
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 164799 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCB301
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Vector typeOomycete expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGFP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer gaattcATGGTGAGCAAGGGCGAG
- 3′ sequencing primer actagtTTACTTGTACAGCTCGTCCATGC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCB301TOR-GFP was a gift from Miaoying Tian (Addgene plasmid # 164799 ; http://n2t.net/addgene:164799 ; RRID:Addgene_164799) -
For your References section:
Establishment of a simple and efficient Agrobacterium-mediated transformation system for Phytophthora palmivora. Wu D, Navet N, Liu Y, Uchida J, Tian M. BMC Microbiol. 2016 Sep 6;16(1):204. doi: 10.1186/s12866-016-0825-1. 10.1186/s12866-016-0825-1 PubMed 27599726
