pCDNA_GFP-mTLN1-FL
(Plasmid
#164838)
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PurposeExpression of GFP tagged FL mouse talin1
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Depositing Lab
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Depositing OrganizationUF Scripps Biomedical Research
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 164838 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 164838-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 164838-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA4.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTLN1
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SpeciesM. musculus (mouse)
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Entrez GeneTln1 (a.k.a. Tln)
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Tag
/ Fusion Protein
- His10-GFP (N terminal on insert)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 164838-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 164838-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA_GFP-mTLN1-FL was a gift from Tina Izard (Addgene plasmid # 164838 ; http://n2t.net/addgene:164838 ; RRID:Addgene_164838) -
For your References section:
A distinct talin2 structure directs isoform specificity in cell adhesion. Rangarajan ES, Primi MC, Colgan LA, Chinthalapudi K, Yasuda R, Izard T. J Biol Chem. 2020 Sep 11;295(37):12885-12899. doi: 10.1074/jbc.RA119.010789. Epub 2020 Jun 30. 10.1074/jbc.RA119.010789 PubMed 32605925
