Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16485||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
The pSAR-MT inducible
expression plasmid was constructed as follows. Two
oligonucleotides (5'-GATCTCGAGCTCCCTGCA-3' and 5'-GGGAGCTCGA-3') were annealed, and the resulting fragment was cloned into the BamHI and PstI sites of the scaffold attachment region (SAR)-containing plasmid p8. The resulting plasmid, pJM7, consisted of two SAR sequences
flanking a short polylinker containing restriction sites for Xhol and PstI. The mutant metallothionein (MT) promoter was amplified by PCR and cloned into pCEP4, linearized
with BglII and NotI, yielding pCEP-MT. A SalI-BamHI
fragment containing an intron from the globin gene was then
inserted into the XhoI and BamHI sites of pCEP-MT, yielding pCEP-MTi. Finally, the MT promoter/intron/poly(A) region was removed from pCEP-MTi by digestion with Sall and cloned into the XhoI site of pJM7, yielding pSAR-MT.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSAR-MT was a gift from Bert Vogelstein (Addgene plasmid # 16485 ; http://n2t.net/addgene:16485 ; RRID:Addgene_16485)
For your References section:Apoptosis and APC in colorectal tumorigenesis. Morin PJ, Vogelstein B, Kinzler KW. Proc Natl Acad Sci U S A. 1996 Jul 23. 93(15):7950-4. 10.1073/pnas.93.15.7950 PubMed 8755583
Map uploaded by the depositor.