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pCEP4 Pig4
(Plasmid #16498)


Item Catalog # Description Quantity Price (USD)
Plasmid 16498 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 10400
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
    serum amyloid A-1 protein preproprotein
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    SAA1 (a.k.a. PIG4, SAA, SAA2, TP53I4)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA tag variant - YAYDVPDYA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EBV-rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that BamHI is not a unique cloning site and is present at bp# 405 & 859.

Compared to the Pig4 reference sequence M10906 provided in Table 1 of the associated publication, the Pig4 insert in this plasmid contains K2E, V70A and A75V mutations.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEP4 Pig4 was a gift from Bert Vogelstein (Addgene plasmid # 16498 ; ; RRID:Addgene_16498)
  • For your References section:

    A model for p53-induced apoptosis. Polyak K, Xia Y, Zweier JL, Kinzler KW, Vogelstein B. Nature. 1997 Sep 18. 389(6648):300-5. 10.1038/38525 PubMed 9305847