pCEP4 Pig4
(Plasmid #16498)

• Depositing Lab: Bert Vogelstein
• Publication: Polyak et al Nature. 1997 Sep 18. 389(6648):300-5.

Backbone

• Vector backbone: pCEP4
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 10400
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Pig4
• Alt name: SAA1
• Alt name: serum amyloid A-1 protein preproprotein
• Species: H. sapiens (human)
• Insert Size (bp): 450
• Entrez Gene: SAA1 (a.k.a. PIG4, SAA, SAA2, TP53I4)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA tag variant - YAYDVPDYA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EBV-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that BamHI is not a unique cloning site and is present at bp# 405 & 859.

Compared to the Pig4 reference sequence M10906 provided in Table 1 of the associated publication, the Pig4 insert in this plasmid contains K2E, V70A and A75V mutations.