pBR322 14-3-3 Promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16517||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4361
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert name14-3-3 promoter
Alt name14-3-3 sigma
SpeciesH. sapiens (human)
Insert Size (bp)9500
Entrez GeneSFN (a.k.a. YWHAS)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer na
- 3′ sequencing primer pBRrevBam (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBR322 14-3-3 Promoter was a gift from Bert Vogelstein (Addgene plasmid # 16517 ; http://n2t.net/addgene:16517 ; RRID:Addgene_16517)
For your References section:14-3-3 sigma is a p53-regulated inhibitor of G2/M progression. Hermeking H, Lengauer C, Polyak K, He TC, Zhang L, Thiagalingam S, Kinzler KW, Vogelstein B. Mol Cell. 1997 Dec . 1(1):3-11. 10.1016/S1097-2765(00)80002-7 PubMed 9659898
Map uploaded by the depositor.