pHRCMV 14-3-3 sigma
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16552||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerVogelstein Lab
- Backbone size w/o insert (bp) 7462
Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name14-3-3 sigma
SpeciesH. sapiens (human)
Insert Size (bp)849
Entrez GeneSFN (a.k.a. YWHAS)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site EcoRV (destroyed during cloning)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHRCMV 14-3-3 sigma was a gift from Bert Vogelstein (Addgene plasmid # 16552 ; http://n2t.net/addgene:16552 ; RRID:Addgene_16552)
For your References section:14-3-3 sigma is a p53-regulated inhibitor of G2/M progression. Hermeking H, Lengauer C, Polyak K, He TC, Zhang L, Thiagalingam S, Kinzler KW, Vogelstein B. Mol Cell. 1997 Dec . 1(1):3-11. 10.1016/S1097-2765(00)80002-7 PubMed 9659898
Map uploaded by the depositor.