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mNP-Luc (PPARdelta promoter)
(Plasmid #16532)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16532 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBV-Luc
  • Backbone manufacturer
    Vogelstein Lab
  • Backbone size w/o insert (bp) 4867
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PPAR delta promoter NP
  • Alt name
    PPAR delta
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    800
  • Mutation
    Mutant binding sites
  • Entrez Gene
    PPARD (a.k.a. FAAR, NR1C2, NUC1, NUCI, NUCII, PPARB)
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site PvuII (unknown if destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer LucNrev
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

mNP represents fragment NP with both potential Tcf-4-binding sites mutated. TRE1 and TRE2 contain four repeats of the two Tcf-4-binding sites, respectively. mTRE1 and mTRE2 are mutant forms of TRE1 and TRE2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mNP-Luc (PPARdelta promoter) was a gift from Bert Vogelstein (Addgene plasmid # 16532 ; http://n2t.net/addgene:16532 ; RRID:Addgene_16532)
  • For your References section:

    PPARdelta is an APC-regulated target of nonsteroidal anti-inflammatory drugs. He TC, Chan TA, Vogelstein B, Kinzler KW. Cell. 1999 Oct 29. 99(3):335-45. 10.1016/S0092-8674(00)81664-5 PubMed 10555149