pRSFDuet-1(nsp8-nsp7)(nsp12) Citations (4)
Originally described in: Fast and efficient purification of SARS-CoV-2 RNA dependent RNA polymerase complex expressed in Escherichia coli.Madru C, Tekpinar AD, Rosario S, Czernecki D, Brule S, Sauguet L, Delarue M PLoS One. 2021 Apr 29;16(4):e0250610. doi: 10.1371/journal.pone.0250610. eCollection 2021. PubMed Journal
Articles Citing pRSFDuet-1(nsp8-nsp7)(nsp12)
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Selenoprotein S Interacts with the Replication and Transcription Complex of SARS-CoV-2 by Binding nsp7.
Ghelichkhani F, Gonzalez FA, Kapitonova MA, Rozovsky S.
J Mol Biol. 2023 Apr 15;435(8):168008. doi: 10.1016/j.jmb.2023.168008. Epub 2023 Feb 10.
PubMed
Associated Plasmids |
| Incipient functional SARS-CoV-2 diversification identified through neural network haplotype maps. Delgado S, Somovilla P, Ferrer-Orta C, Martinez-Gonzalez B, Vazquez-Monteagudo S, Munoz-Flores J, Soria ME, Garcia-Crespo C, de Avila AI, Duran-Pastor A, Gadea I, Lopez-Galindez C, Moran F, Lorenzo-Redondo R, Verdaguer N, Perales C, Domingo E. Proc Natl Acad Sci U S A. 2024 Mar 5;121(10):e2317851121. doi: 10.1073/pnas.2317851121. Epub 2024 Feb 28. PubMed |
| Drug repurposing screen to identify inhibitors of the RNA polymerase (nsp12) and helicase (nsp13) from SARS-CoV-2 replication and transcription complex. Kuzikov M, Reinshagen J, Wycisk K, Corona A, Esposito F, Malune P, Manelfi C, Iaconis D, Beccari A, Tramontano E, Nowotny M, Windshugel B, Gribbon P, Zaliani A. Virus Res. 2024 May;343:199356. doi: 10.1016/j.virusres.2024.199356. Epub 2024 Mar 16. PubMed |
| Point mutations at specific sites of the nsp12-nsp8 interface dramatically affect the RNA polymerization activity of SARS-CoV-2. Ferrer-Orta C, Vazquez-Monteagudo S, Ferrero DS, Martinez-Gonzalez B, Perales C, Domingo E, Verdaguer N. Proc Natl Acad Sci U S A. 2024 Jul 16;121(29):e2317977121. doi: 10.1073/pnas.2317977121. Epub 2024 Jul 11. PubMed |
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