Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16573||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneCDK4 (a.k.a. CMM3, PSK-J3)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site SalI (destroyed during cloning)
- 5′ sequencing primer MSCV (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The CDK4 ORF was generated by PCR using the expressed sequence tag W77860 as a template and the primers 5'-GCGGATCCGC GGCCGCCTTC CACCATGGCT ACCTCTCGAT CTGAGC-3' and 5'-CGGTCGACTC ACTCCGGATT ACCTTCATC-3'. The resulting product was digested with the enzymes NotI and SalI and inserted into the respective sites of the vector G1BgSVNA (a retroviral vector encoding a hygromycin resistance gene and beta-galactosidase).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:G1BgSVNA-CDK4 was a gift from Bert Vogelstein (Addgene plasmid # 16573 ; http://n2t.net/addgene:16573 ; RRID:Addgene_16573)
For your References section:Identification of CDK4 as a target of c-MYC. Hermeking H, Rago C, Schuhmacher M, Li Q, Barrett JF, Obaya AJ, O'Connell BC, Mateyak MK, Tam W, Kohlhuber F, Dang CV, Sedivy JM, Eick D, Vogelstein B, Kinzler KW. Proc Natl Acad Sci U S A. 2000 Feb 29. 97(5):2229-34. 10.1073/pnas.050586197 PubMed 10688915
Map uploaded by the depositor.