G1BgSVNA-CDK4
(Plasmid #16573)

• Depositing Lab: Bert Vogelstein
• Publication: Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 97(5):2229-34.

Backbone

• Vector backbone: G1BgSVNA
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CDK4
• Species: H. sapiens (human)
• Entrez Gene: CDK4 (a.k.a. CMM3, PSK-J3)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: MSCV

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The CDK4 ORF was generated by PCR using the expressed sequence tag W77860 as a template and the primers 5'-GCGGATCCGC GGCCGCCTTC CACCATGGCT ACCTCTCGAT CTGAGC-3' and 5'-CGGTCGACTC ACTCCGGATT ACCTTCATC-3'. The resulting product was digested with the enzymes NotI and SalI and inserted into the respective sites of the vector G1BgSVNA (a retroviral vector encoding a hygromycin resistance gene and beta-galactosidase).