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Addgene

pBGE2.0
(Plasmid #165987)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 165987 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pPtGE31
  • Backbone manufacturer
    Slattery et al., 2018
  • Backbone size w/o insert (bp) 9507
  • Total vector size (bp) 18639
  • Modifications to backbone
    Addition of elements for S. meliloti replication and selection.
  • Vector type
    Synthetic Biology ; Bacterial and Yeast Cloning; Conjugation
  • Selectable markers
    HIS3 ; Chloramphenicol - E. coli, Tetracycline - S. meliloti, Nourseothricin N-acetyltransferase - P. tricornutum

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    E. coli Epi300
  • Growth instructions
    Plasmid can be induced to high copy with arabinose.
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    RK2/RP4 origin of transfer
  • Alt name
    oriT
  • Insert Size (bp)
    771

Gene/Insert 2

  • Gene/Insert name
    repA1B1C1
  • Species
    Sinorhizobium meliloti
  • Insert Size (bp)
    4497

Gene/Insert 3

  • Gene/Insert name
    nourseothricin N-acetyl transferase
  • Alt name
    NAT
  • Insert Size (bp)
    570

Gene/Insert 4

  • Gene/Insert name
    tetracycline resistance
  • Alt name
    TetR/A
  • Insert Size (bp)
    2285

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    (1) Edgell Lab (backbone was cloned from pPtGE30). Reference: Slattery, S. S., Diamond, A., Wang, H., Therrien, J. A., Lant, J. T., Jazey, T., ... & Edgell, D. R. (2018). An expanded plasmid-based genetic toolbox enables Cas9 genome editing and stable maintenance of synthetic pathways in Phaeodactylum tricornutum. ACS synthetic biology, 7(2), 328-338. (2) Jones and Gutterson (Tetracycline resistance gene was cloned from pRK7813). Reference: Jones, J. D., & Gutterson, N. (1987). An efficient mobilizable cosmid vector, pRK7813, and its use in a rapid method for marker exchange in Pseudomonas fluorescens strain HV37a. Gene, 61(3), 299-306.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBGE2.0 was a gift from Bogumil Karas (Addgene plasmid # 165987 ; http://n2t.net/addgene:165987 ; RRID:Addgene_165987)
  • For your References section:

    Designer Sinorhizobium meliloti strains and multi-functional vectors enable direct inter-kingdom DNA transfer. Brumwell SL, MacLeod MR, Huang T, Cochrane RR, Meaney RS, Zamani M, Matysiakiewicz O, Dan KN, Janakirama P, Edgell DR, Charles TC, Finan TM, Karas BJ. PLoS One. 2019 Jun 17;14(6):e0206781. doi: 10.1371/journal.pone.0206781. eCollection 2019. 10.1371/journal.pone.0206781 PubMed 31206509