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(Plasmid #165992)


Item Catalog # Description Quantity Price (USD)
Plasmid 165992 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5676
  • Total vector size (bp) 6171
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
    Influenza hemagglutinin ministem
  • Species
  • Insert Size (bp)
  • Promoter T7
  • Tags / Fusion Proteins
    • HisTag (N terminal on insert)
    • Foldon (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BlpI (not destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer none
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Original publication:

Mallajosyula VV, Citron M, Ferrara F, Lu X, Callahan C, Heidecker GJ, Sarma SP, Flynn JA, Temperton NJ, Liang X, Varadarajan R. Influenza hemagglutinin stem-fragment immunogen elicits broadly neutralizing antibodies and confers heterologous protection. Proc Natl Acad Sci U S A. 2014 Jun 24;111(25):E2514-23. doi: 10.1073/pnas.1402766111. Epub 2014 Jun 9. PMID: 24927560; PMCID: PMC4078824.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET11b-HA2 was a gift from Vesa Hytönen (Addgene plasmid # 165992 ; ; RRID:Addgene_165992)
  • For your References section:

    Modular vaccine platform based on the norovirus-like particle. Lampinen V, Heinimaki S, Laitinen OH, Pesu M, Hankaniemi MM, Blazevic V, Hytonen VP. J Nanobiotechnology. 2021 Jan 19;19(1):25. doi: 10.1186/s12951-021-00772-0. 10.1186/s12951-021-00772-0 PubMed 33468139