Purpose(Empty Backbone) Bacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||166005||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 1700
Vector typeBacterial Expression, CRISPR
Growth in Bacteria
Copy numberLow Copy
- Cloning method Gibson Cloning
- 5′ sequencing primer CGTCACACTTTGCTATGCCA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Tight inducible control of dCas9 and sgRNA expression in bacteria. sgRNA spacer is cloned in between BsaI sites, replacing an RFP cassette to facilitate identification of successful clones.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pdCas9-sgRNA-RFP was a gift from Martin Buck (Addgene plasmid # 166005 ; http://n2t.net/addgene:166005 ; RRID:Addgene_166005)