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pEGFP_E1770Q-talin1 (1-2541)
(Plasmid #166121)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166121 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 166121-D50 50 μg of DNA in Tris buffer $495
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DNA 166121-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4734
  • Total vector size (bp) 12357
  • Vector type
    Mammalian Expression ; CMV promoter

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For MidiPrep according to the instructions from the kit.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E1770Q-Talin-1
  • Alt name
    E1770Q-TLN1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    7623
  • Mutation
    E1770Q
  • Entrez Gene
    Tln1 (a.k.a. Tln)
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP_E1770Q-talin1 (1-2541) was a gift from Vesa Hytönen (Addgene plasmid # 166121 ; http://n2t.net/addgene:166121 ; RRID:Addgene_166121)

  • For your References section:

    Cancer associated talin point mutations disorganise cell adhesion and migration. Azizi L, Cowell AR, Mykuliak VV, Goult BT, Turkki P, Hytonen VP. Sci Rep. 2021 Jan 11;11(1):347. doi: 10.1038/s41598-020-77911-4. 10.1038/s41598-020-77911-4 PubMed 33431906
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