pAG415GPD-mRuby3
(Plasmid
#166138)
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PurposemRuby3 expression under control of the GPD promoter in yeast
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 166138 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS415
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemRuby3
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SpeciesSynthetic
- Promoter GDP
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2022.02.26.482115 for bioRxiv preprint. Also see Chen and Harel et al. 2024 (https://doi.org/10.1016/j.devcel.2024.04.014)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAG415GPD-mRuby3 was a gift from Daniel Jarosz (Addgene plasmid # 166138 ; http://n2t.net/addgene:166138 ; RRID:Addgene_166138) -
For your References section:
Identification of protein aggregates in the aging vertebrate brain with prion-like and phase-separation properties. Harel I, Chen YR, Ziv I, Singh PP, Heinzer D, Navarro Negredo P, Goshtchevsky U, Wang W, Astre G, Moses E, McKay A, Machado BE, Hebestreit K, Yin S, Sanchez Alvarado A, Jarosz DF, Brunet A. Cell Rep. 2024 Jun 25;43(6):112787. doi: 10.1016/j.celrep.2023.112787. Epub 2024 May 28. 10.1016/j.celrep.2023.112787 PubMed 38810650