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Addgene

pDEST17-His10-ccdB
(Plasmid #166145)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166145 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST17
  • Vector type
    Bacterial Expression
  • Tag / Fusion Protein
    • His10 (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • His10 (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.02.26.482115 for bioRxiv preprint. Also see Chen and Harel et al. 2024 (https://doi.org/10.1016/j.devcel.2024.04.014)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST17-His10-ccdB was a gift from Daniel Jarosz (Addgene plasmid # 166145 ; http://n2t.net/addgene:166145 ; RRID:Addgene_166145)

  • For your References section:

    Identification of protein aggregates in the aging vertebrate brain with prion-like and phase-separation properties. Harel I, Chen YR, Ziv I, Singh PP, Heinzer D, Navarro Negredo P, Goshtchevsky U, Wang W, Astre G, Moses E, McKay A, Machado BE, Hebestreit K, Yin S, Sanchez Alvarado A, Jarosz DF, Brunet A. Cell Rep. 2024 Jun 25;43(6):112787. doi: 10.1016/j.celrep.2023.112787. Epub 2024 May 28. 10.1016/j.celrep.2023.112787 PubMed 38810650
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