pDONR221-DDX5ΔIDR
(Plasmid
#166165)
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PurposeGateway entry vector for N. furzeri DDX5ΔIDR(1-483) mutant
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 166165 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221
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Vector typeGateway Entry Vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDDX5ΔIDR(1-483)
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SpeciesN. furzeri
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Insert Size (bp)1449
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Mutationdeletion of 1450-1902
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Stopless entry clone. Please visit https://doi.org/10.1101/2022.02.26.482115 for bioRxiv preprint. Also see Chen and Harel et al. 2024 (https://doi.org/10.1016/j.devcel.2024.04.014)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR221-DDX5ΔIDR was a gift from Daniel Jarosz (Addgene plasmid # 166165 ; http://n2t.net/addgene:166165 ; RRID:Addgene_166165) -
For your References section:
Identification of protein aggregates in the aging vertebrate brain with prion-like and phase-separation properties. Harel I, Chen YR, Ziv I, Singh PP, Heinzer D, Navarro Negredo P, Goshtchevsky U, Wang W, Astre G, Moses E, McKay A, Machado BE, Hebestreit K, Yin S, Sanchez Alvarado A, Jarosz DF, Brunet A. Cell Rep. 2024 Jun 25;43(6):112787. doi: 10.1016/j.celrep.2023.112787. Epub 2024 May 28. 10.1016/j.celrep.2023.112787 PubMed 38810650