pDONR221-SLC25A19
(Plasmid
#166209)
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PurposeGateway entry vector for N. furzeri SLC25A19
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 166209 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221
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Vector typeGateway Entry Vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSLC25A19
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SpeciesN. furzeri
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Insert Size (bp)996
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Stopless entry clone. Please visit https://doi.org/10.1101/2022.02.26.482120 for bioRxiv preprint. Also see Harel and Chen et al. 2024 (https://doi.org/10.1016/j.celrep.2023.112787)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR221-SLC25A19 was a gift from Daniel Jarosz (Addgene plasmid # 166209 ; http://n2t.net/addgene:166209 ; RRID:Addgene_166209) -
For your References section:
Tissue-specific landscape of protein aggregation and quality control in an aging vertebrate. Chen YR, Harel I, Singh PP, Ziv I, Moses E, Goshtchevsky U, Machado BE, Brunet A, Jarosz DF. Dev Cell. 2024 Jul 22;59(14):1892-1911.e13. doi: 10.1016/j.devcel.2024.04.014. Epub 2024 May 28. 10.1016/j.devcel.2024.04.014 PubMed 38810654