Purposeregulated dCas9 generator
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||166245||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 2603
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Growth Strain(s)NEB Stable
Growth instructionsTo successfully obtain the bacterial transformants of this plasmid, it required to use pAUX_OL plasmid.
Copy numberHigh Copy
Insert Size (bp)4574
- Promoter Ec-TTL-P112 and Ec-TTL-P104
- Cloning method Gibson Cloning
- 5′ sequencing primer GGCGCGCCAAAAAGAGTATTG
- 3′ sequencing primer tataaacgcagaaaggcccacc (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pdCas9_CL was a gift from Domitilla Del Vecchio (Addgene plasmid # 166245 ; http://n2t.net/addgene:166245 ; RRID:Addgene_166245)
For your References section:dCas9 regulator to neutralize competition in CRISPRi circuits. Huang HH, Bellato M, Qian Y, Cardenas P, Pasotti L, Magni P, Del Vecchio D. Nat Commun. 2021 Mar 16;12(1):1692. doi: 10.1038/s41467-021-21772-6. 10.1038/s41467-021-21772-6 PubMed 33727557