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Addgene

Cdc42 T17N pCS2
(Plasmid #16670)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16670 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCS2 (modified)
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Vertebrate

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cdc42 T17N
  • Alt name
    Cdc42
  • Species
    H. sapiens (human)
  • Mutation
    T17N. Dominant negative.
  • Entrez Gene
    CDC42 (a.k.a. CDC42Hs, G25K, TKS)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This cDNA encodes a dominant negative version of Cdc42, a Rho GTPase.

The vector is pCS2, but note the SP6 PROMOTER IS ABSENT in this construct. Thus use for DNA injection/transfection relying on the CMV promoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cdc42 T17N pCS2 was a gift from De-Li Shi (Addgene plasmid # 16670 ; http://n2t.net/addgene:16670 ; RRID:Addgene_16670)
  • For your References section:

    Role of frizzled 7 in the regulation of convergent extension movements during gastrulation in Xenopus laevis. Djiane A, Riou J, Umbhauer M, Boucaut J, Shi D. Development. 2000 Jul . 127(14):3091-100. PubMed 10862746