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Cdc42 T17N sp64T
(Plasmid #16672)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16672 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSP64T
  • Backbone manufacturer
    Available at Addgene (#15030)
  • Backbone size w/o insert (bp) 3300
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cdc42 T17N
  • Alt name
    Cdc42
  • Species
    H. sapiens (human)
  • Mutation
    T17N. Dominant negative.
  • Entrez Gene
    CDC42 (a.k.a. CDC42Hs, G25K, TKS)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

This cDNA encodes a dominant negative version of Cdc42, a Rho GTPase.

The vector is pSP64T, which allows in vitro transcription from the SP6 promoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cdc42 T17N sp64T was a gift from De-Li Shi (Addgene plasmid # 16672 ; http://n2t.net/addgene:16672 ; RRID:Addgene_16672)
  • For your References section:

    Role of frizzled 7 in the regulation of convergent extension movements during gastrulation in Xenopus laevis. Djiane A, Riou J, Umbhauer M, Boucaut J, Shi D. Development. 2000 Jul . 127(14):3091-100. PubMed 10862746