Tg(mpeg1:LifeAct-mRuby)
(Plasmid #166977)

• Purpose: labels F-actin in macrophages
• Depositing Lab: Anna Huttenlocher
• Publication: Barros-Becker et al J Cell Sci. 2017 Nov 15;130(22):3801-3808. doi: 10.1242/jcs.206128. Epub 2017 Sep 28.

Backbone

• Vector backbone: Tol2
• Backbone size w/o insert (bp): 3500
• Total vector size (bp): 6507

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: mpeg1
• Species: D. rerio (zebrafish)
• Insert Size (bp): 1854
• Entrez Gene: mpeg1.1 (a.k.a. fj09e08, mpeg, mpeg1, wu:fb14d06, wu:fj09e08, zgc:66409)
• Tag / Fusion Protein:
  • Lifeact-mRuby (N terminal on backbone)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: xhoI (not destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: gtgaaagtacaa

Gene/Insert 2

• Gene/Insert name: LifeAct
• Species: X. laevis (frog)
• Entrez Gene: actb1 (a.k.a. ACTB, B-ACTZF, actba, bact, bactin1, bactzf, wu:fd18f05)
• Tag / Fusion Protein:
  • mRuby (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: xbaI (not destroyed)
• 5′ sequencing primer: TGCAGGAGACAGCAAAAg
• 3′ sequencing primer: ccagaggtgtaa

Resource Information

• A portion of this plasmid was derived from a plasmid made by: LifeAct was a gift from Dr Bill Bement (University of WI-Madison)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry