|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45246||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 12600
Modifications to backboneadded pRuby tagged LifeAct
Vector typezebrafish expression
Growth in Bacteria
- Promoter mpx
- Cloning method Restriction Enzyme
- 5′ cloning site bamHI (destroyed during cloning)
- 3′ cloning site salI (not destroyed)
- 5′ sequencing primer ccaggtcattgcacaacaccag
- 3′ sequencing primer gttatccgctcacaattccacac (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bythe original plasmid is a generous gift from M. Sixt
Terms and Licenses
- Not Available to Industry
Article Citing this Plasmid
Please note that Addgene's sequencing results differ from the full plasmid sequence information from the depositing laboratory at bp# 9886. This difference is in the vector backbone and is not a concern for the function of the plasmid according to the depositing laboratory.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Tol2-mpx:Lifeact-Ruby was a gift from Anna Huttenlocher (Addgene plasmid # 45246 ; http://n2t.net/addgene:45246 ; RRID:Addgene_45246)
For your References section:Differential regulation of protrusion and polarity by PI3K during neutrophil motility in live zebrafish. Yoo SK, Deng Q, Cavnar PJ, Wu YI, Hahn KM, Huttenlocher A. Dev Cell. 2010 Feb 16. 18(2):226-36. 10.1016/j.devcel.2009.11.015 PubMed 20159593