tol2-4xnr UAS-PA Rac1-mCherry
(Plasmid #41878)

• Purpose: Tol2 transgenesis to express photoactivatable Rac1 under 4xnr UAS promoter
• Depositing Lab: Anna Huttenlocher
• Depositing Organization: University of Wisconsin, Madison
  Located in United States of America
• Publication: tol2-4xnrUAS:PA Rac1-mcherry (unpublished)
• Citations: 2 Articles

Backbone

• Vector backbone: Tol2
• Total vector size (bp): 5473
• Vector type: Zebrafish expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: photoactivatable Rac1
• Alt name: PA-Rac1
• Species: H. sapiens (human)
• Mutation: Rac1 starts at I4 and contains mutations Q61L, E91H and N92H
• Entrez Gene: RAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
• Promoter: 4xnr UAS
• Tags / Fusion Proteins:
  • 6x His (N terminal on insert)
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: PA-Rac1 from Klaus Hahn (Addgene Plasmid #22027)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results differ from the full plasmid sequence provided by the depositing laboratory at bp# 895, 920 and 1702. According to the depositing lab, these differences are not a concern for the function of the plasmid.