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(Plasmid #167118)


Item Catalog # Description Quantity Price (USD)
Plasmid 167118 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 3800
  • Total vector size (bp) 4710
  • Modifications to backbone
    removal of CddB-Cm resistance cassette. Introduction Kpn1-3xFlag-Not1-MEFVp.S242R-Xho1
  • Vector type
    gateway entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    H. sapiens (human)
  • Mutation
    M694V Methionine 694 to valine (FMF mutation)
  • Entrez Gene
    MEFV (a.k.a. FMF, MEF, PAAND, TRIM20)
  • Tag / Fusion Protein
    • 3xFlag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not1 (not destroyed)
  • 3′ cloning site xho1 (not destroyed)
  • 5′ sequencing primer TAGTTACTTAAGCTCGGGCCCC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR1A-3xFlag-M694V-MEFV was a gift from Thomas Henry (Addgene plasmid # 167118 ; ; RRID:Addgene_167118)