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(Plasmid #167126)


Item Catalog # Description Quantity Price (USD)
Plasmid 167126 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5442
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    SARS-CoV-2 Receptor Binding domain
  • Species
  • Insert Size (bp)
  • Mutation
    residues (333 to 529 aa)
  • GenBank ID
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 Fwd
  • 3′ sequencing primer T7 Term
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    cloned from Addgene

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21b-RBD-JA1 was a gift from David Margulies (Addgene plasmid # 167126 ; ; RRID:Addgene_167126)
  • For your References section:

    Synthetic nanobody-SARS-CoV-2 receptor-binding domain structures identify distinct epitopes. Ahmad J, Jiang J, Boyd LF, Natarajan K, Margulies DH. bioRxiv. 2021 Jan 27. doi: 10.1101/2021.01.27.428466. 10.1101/2021.01.27.428466 PubMed 33532775