Purpose(Empty Backbone) Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mClover3 and selection with Puromycin
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||167205||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size (bp) 2458
Vector typeMammalian Expression, CRISPR
/ Fusion Protein
- mClover3 (C terminal on backbone)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth instructionsThe empty plasmid must be maintained in E.coli DB3.1. Once the plasmid is used to insert homologous recombination arms, the plasmid must be transformed in a strain that is sensitive to ccdb such as DH5Alpha or similar.
Copy numberHigh Copy
- Cloning method Gibson Cloning
- 5′ sequencing primer gcagattgtactgagagtgcaccata
- 3′ sequencing primer caggttttgctttttggcctttccc (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FASTHDR-Cterm-mClover3-Puromycin was a gift from Oscar Perez (Addgene plasmid # 167205 ; http://n2t.net/addgene:167205 ; RRID:Addgene_167205)
For your References section:Multiplex Gene Tagging with CRISPR-Cas9 for Live-Cell Microscopy and Application to Study the Role of SARS-CoV-2 Proteins in Autophagy, Mitochondrial Dynamics, and Cell Growth. Perez-Leal O, Nixon-Abell J, Barrero CA, Gordon JC, Oesterling J, Rico MC. CRISPR J. 2021 Nov 30. doi: 10.1089/crispr.2021.0041. 10.1089/crispr.2021.0041 PubMed 34847745