pInducer(p20) x mKate2-NLS-P2A-HRas(N17)_ires-puro
(Plasmid #167832)

• Purpose: Inducible expression of dominant negative HRas(N17) and P2A-coupled reporter mKate2-NLS
• Depositing Lab: Hugo Snippert
• Publication: Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. doi: 10.1038/s41556-021-00654-5. Epub 2021 Apr 1.

Backbone

• Vector backbone: pInducer20
• Backbone manufacturer: Meerbey et al., PNAS
• Backbone size w/o insert (bp): 5900
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin ; mKate2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HRAS(N17)
• Species: H. sapiens (human)
• Insert Size (bp): 570
• Mutation: S17N
• Entrez Gene: HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
• Promoter: Ubc, TRE

Cloning Information

• Cloning method: Ligation Independent Cloning

Resource Information

• Supplemental Documents:
  • pInducer(p20) x mKate2-NLS-P2A-HRas(N17)_ires-puro.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments