Bin1 N-BAR domain
(Plasmid
#168280)
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PurposeExpress N-BAR domain of Bin1 (aa 1-267) in pEGFP-N1 vector
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Depositing Lab
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Depositing OrganizationUniversity of Hong Kong
Located in Hong Kong Special Administrative Region of China -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 168280 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 168280-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 168280-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFP-N1
- Backbone size w/o insert (bp) 4733
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBin1
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Alt nameBridging integrator 1
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SpeciesM. musculus (mouse)
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Insert Size (bp)801
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Mutationaa 1-267
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GenBank IDNM_001083334.1
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Entrez GeneBin1 (a.k.a. ALP-1, Am, Amphl, BRAMP-2, SH3P9)
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Tag
/ Fusion Protein
- EGFP (C terminal on backbone)
Resource Information
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A portion of this plasmid was derived from a plasmid made byWildtype Bin1 plasmid was from Addgene 27693.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 168280-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 168280-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Bin1 N-BAR domain was a gift from Cheng-han Yu (Addgene plasmid # 168280 ; http://n2t.net/addgene:168280 ; RRID:Addgene_168280) -
For your References section:
Podosome formation promotes plasma membrane invagination and integrin-beta3 endocytosis on a viscous RGD-membrane. Cao F, Zhou Y, Liu X, Yu CH. Commun Biol. 2020 Mar 13;3(1):117. doi: 10.1038/s42003-020-0843-2. 10.1038/s42003-020-0843-2 PubMed 32170110
