Bin1 delta N-BAR
(Plasmid
#168282)
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PurposeExpress N-BAR-deleted Bin1 mutant (aa 268-476)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 168282 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepmCherry-N1
- Backbone size w/o insert (bp) 4733
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBin1
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Alt nameBridging integrator 1
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SpeciesM. musculus (mouse)
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Insert Size (bp)627
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Mutationaa 268-476
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GenBank IDNM_001083334.1
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Entrez GeneBin1 (a.k.a. ALP-1, Am, Amphl, BRAMP-2, SH3P9)
Resource Information
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A portion of this plasmid was derived from a plasmid made byWildtype Bin1 plasmid was from Addgene 27693, and mCherry tag was removed during the subcloning.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Bin1 delta N-BAR was a gift from Cheng-han Yu (Addgene plasmid # 168282 ; http://n2t.net/addgene:168282 ; RRID:Addgene_168282) -
For your References section:
Podosome formation promotes plasma membrane invagination and integrin-beta3 endocytosis on a viscous RGD-membrane. Cao F, Zhou Y, Liu X, Yu CH. Commun Biol. 2020 Mar 13;3(1):117. doi: 10.1038/s42003-020-0843-2. 10.1038/s42003-020-0843-2 PubMed 32170110
